René Lesnik

3 Questions 5 Answers 0 Followers

Questions related from René Lesnik

Does anyone have a protocol for DNA extraction from MicroGard bacterial/viral filters?

We have a collection of inoculated MicroGard filters (see link) and we would like to extract DNA from those. Thus, we are looking for a good procedure to do so. We have experience in extracting...

24 September 2014 3,222 0 View

Why does my amplification of ssDNA fragments not work?

Hey guys, I have separated ssDNA fragments (purified from dsDNA PCR amplicons by Lamda-exonuclease digestion) by SSCP (basically a PAGE assay) and want to reamplify the single bands of the SSCP...

17 September 2014 8,489 4 View

What's your favorite method to extract total RNA from environmental biofilm swab samples?

Hi, I want to extract RNA from environmental biofilms (water distribution systems) and I am looking for a good protocol. We use FLOQSwabs(TM) and the Fast DNA Spin Kit for soil from MP Bio to...

15 July 2014 2,970 2 View