Which solvents are useful to dissolve liposomes (lipid vesicles) with protein without precipitating the protein?

Hi. I'm encapsulating a protein inside a liposome (lipid vesicle). I want to quantify encapsulated protein by UV since it absorbs and the lipids don't. But I need to dissolve both the liposomes...

04 April 2019 3,719 10 View

Which solvents are useful to dissolve liposomes (lipid vesicles) with protein without precipitating the protein?

Hi. I'm encapsulating a protein inside a liposome (lipid vesicle). I want to quantify encapsulated protein by UV since it absorbs and the lipids don't. But I need to dissolve both the liposomes...

03 April 2019 2,947 8 View

What would be the best approach to perform a determination of curcumin in mice organs (biodistribution assay) by fluorescence spectroscopy?

07 August 2018 517 2 View

What would be the best approach to perform a determination of curcumin in mice organs (biodistribution assay) by fluorescence spectroscopy?

03 August 2018 898 2 View

What is the best cationic material/compound to stabilize a drug nanocrystal dispersion for subcutaneous delivery?

I've been searching for cationic materials to stabilize a suspension of drug nanocrystals. We want to prepare curcumin nanocrystals (with a bottom up approach, probably) but we need them to be...

03 April 2018 6,811 5 View

What is the effect of the polydispersity (size particle distribution) on the stability of liposomes?

In other words: is there an effect on the stability when comparing highly monodisperse liposomes (for example 100 nm +/- 5) versus highly polydisperse liposomes (for example 100 nm +/- 50 nm? Is...

10 November 2017 6,414 6 View

Can solvents used in histopathology dissolve substances that I need to observe in a specific mice tissue?

01 February 2016 6,487 5 View

Can solvents used in histopathology dissolve substances that I need to observe in a specific mice tissue?

01 February 2016 8,549 4 View

Which are the best methodologies to extract protein from liposomes?

We are trying to quantify protein encapsulated in liposomes. Since lipids in BCA interfere with the measurement, we need to perform a extraction and get rid off the lipids but with a good recovery...

01 January 1970 5,354 5 View

Is cytotoxicity of stearylamine bearing liposomes related to the molecule or to the zeta potential?

I'm preparing cationic liposomes in order to perform in vitro cell assays, so we need to test cytotoxicity first. But I'm not sure if the problem is stearylamine as itself or a high zeta...

01 January 1970 9,415 10 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

Can COVID-19 vaccines protect against the emerging strains of SARS-CoV-2?

Can COVID-19 vaccines protect against the emerging strains of SARS-CoV-2?

18 February 2021 8,221 8 View