I want to enclose a 6 kbp plasmid DNA into nanoparticles. But the nanoparticles I use is about 30 nm in diameter. I wonder whether they can hold the DNA. Can someone give some advice? Thanks!
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Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
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Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
We invite qualified candidates of Visiting Scholars to engage in research collaboration or deliver lectures on their research, with travel expenses covered. The visiting period is flexible,...
20 July 2024 1,165 1 View
Although I have extensive experience in dental implants and periodontal treatment, I recognize that we face some deficiencies in my clinic. First, we need to update our equipment, especially in...
09 July 2024 2,004 3 View
This research question explores the complex relationship between bacterial diversity in the oral microenvironment and the clinical manifestations of periodontal disease. The oral microenvironment...
09 July 2024 6,210 1 View
Scholars from multiple disciplines stressed the importance of AI Technology in ESL teaching from a theoretical perspective. Are there teaching designs that combine theoretical discussions and...
01 July 2024 999 7 View
For the measurement of electrode reaction kinetics and electron transfer rate constant, using E. Laviron's model and RICHARD S. Nicholson's model, the greater peak potential separation indicates...
30 June 2024 580 3 View
Dear colleagues, I am currently using HCT-116 adherent cells for cell culture in 96-well plates and have some questions regarding cell counting. I have tried various methods and found the most...
22 June 2024 1,124 5 View
Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...
05 August 2024 8,946 3 View
Some scientists use nano nitrogen and some use nano urea, even though the process used the same for preparation. So, which is true nano nitrogen or nano urea?
28 June 2024 2,885 6 View
Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...
20 June 2024 9,587 1 View
I am working with chitosan/dextran sulphate nanoparticles loaded with rifampicin, which is fluorescent. Dsl analysis are bad and I saw that is necessary use a narrow band filter. How can I select...
05 June 2024 1,324 3 View
nano additives and lubricants for hybrid electric vehicles
24 May 2024 8,363 3 View
I have prepared some polymeric microspheres from chitosan and PCL. However while performing in vitro degradation study, I found difficulties in both above mentioned methods. Here I am mentioning...
20 May 2024 6,114 4 View
can we make aqueous or ethanol extraction or by other solvent extraction in Nano size? which method that used for it if possible, please?
19 May 2024 9,308 1 View
Hi everyone, I'm currently working on a project where we are using a Jetson Nano in a car, supported by a Waveshare UPS Module B for battery backup. To charge the UPS Module, we need a supply of...
19 May 2024 205 3 View
TG measurements on the Hitachi STA7300 equipment started to have different results for the same material after some time of use Some tests have already been carried out to try to correct it: Gas...
17 May 2024 7,438 4 View
to be involved in our research please massage me here
15 May 2024 8,998 2 View