I used Hep III (from Sigma, reconstituted using manufacturer's protocol) and incubated the lysates with Hep III (final concentration 2U/ml, 4h at 37). But this gives me precipitate at the end of the incubation period. I also lose significant amount of protein post treatment.
Dear Indrayani Waghmare
Pls. find the attached files
http://www.nature.com/articles/srep21410
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2886746/
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0049092
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178971/
https://www.neb.com/products/glycobiology/heparinases/heparinases
http://www.sciencedirect.com/science/article/pii/S1074552111002080
http://www.jbc.org/content/283/41/27724.short
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3019558/
http://gut.bmj.com/content/early/2016/09/08/gutjnl-2015-311289.full.pdf
Hoping this will be helpful
Regards
Prof. Houda Kawas
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