Can anyone provide me a protocl of Native PAGE with the 30% terminal resolving gel concentraion?

I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat...

22 January 2021 8,522 3 View

Naming the "UK coronavirus variant" rises to the occasion?

In the context of the COVID-19 infodemic, the global profusion of monikers and hashtags for "UK coronavirus variant" have found their way into daily communication and contributed to a backlash...

01 January 2021 7,850 2 View

Number of Estimating Equations Required to Test Hypotheses?

After literature review, current draft as below: Variables: Independent = B (represented by I, M, O, S, C), Moderating = W Dependent = F Control = CV Hypotheses: H1a. I has significant...

18 September 2020 7,055 2 View

Why is my protein a smear when I detect with CoIP lysis buffer plus LDS?

Hi I am trying to express my protein R in tobacco leaves and do a CoIP, but I can't detect the protein R in CoIP input. I took the same tissue and added 8M Urea plus LDS protein buffer, protein R...

31 August 2020 9,534 10 View

What's the main difference between #Tris-glycine #SDS-PAGE and #Bis-Tris #SDS-PAGE. How pH and ion strenght affect SDS-PAGE?

sometimes, there is some smear on the SDS-PAGE, this can be eliminated with a different running buffer or a different PAGE, or a different way of sample loading buffer. how these affect the SDS-PAGE?

09 August 2020 6,149 3 View

Is there a gentle method to reduce cuprous oxide to copper while maintaining the original morphology?

Hello, Recently, I have synthesized some cuprous oxide nanomaterials and want to reduce them to metal while maintaining the morphology. Is there a way to do this?

04 August 2020 4,850 3 View

Is there a function coded by matlab to reproject the geographic raster data?

Is there a function coded by matlab to reproject the geographic raster data?especially for the case converting the Ease-Grid 2.0 to the geographic coordinate.

17 June 2020 7,875 2 View

Why the contaminated colony grow around the plate?

Doing the antibacterial test, the unknown colony grows around the plate. E. coli and antibacterial regent were mixed with LB, PBS and EDTA. Not likely the contamination was from the LB, PBS or...

18 May 2020 1,311 3 View

How to do a two-way ONOVA in SPSS?

I don't have advanced knowledge of SPSS and tutorials aren't making much sense to me, if anyone is able to explain SPSS two-way ONOVA step-by-step in the simplest form I would be grateful. My...

30 March 2020 8,769 2 View

Statistical tests for dummies - one way or two way onova?

I'm currently working on my under-grad dissertation project, researching whether Raffinose impacts gut barrier function. I have used a control measure and a high, medium and low dose of Raffinose...

24 March 2020 4,220 6 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Solution of 2D convection diffusion heat equation using MATLAB ?

This is a polymer heating problem. Polymer enters at around 180C (x=0) in the barrel. Also barrel initial temperature is 180 (t=0). Some heat Is added along whole length of barrel q. We have to...

15 February 2021 3,887 1 View

How can I spin a thick layer of organic polymer?

I need to spin a 120um thick film. My current procedure consists of spinning the polymer (about 20um), uv exposure, plasma etch, and then spinning another 20um thick layer, uv again, plasma etch...

10 February 2021 2,055 6 View

Defining effective diffusivity in porous zone and non-porous zone?

Hi all, I am working on methanol steam reforming using packed bed for hydrogen generation. I am using FLUENT software for CFD simulation. In reference paper, authors mentioned that effective mass...

10 February 2021 6,104 1 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Is it possible to have a diffusion of oxygen from the glass substrate (SiO2) into the absorbent layer during annealing process?

02 February 2021 382 7 View

How to apply graphene oxide on an gold electrode?

Dear Fellows, now it is the time to push graphene applications further in terms of easy and low cost production surroundings. Thus I recently dived in the materia and I am facing tons of papers,...

02 February 2021 6,284 2 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

How to make a PEDOT:PSS film by spin coating?

Hi,I'm a student who is studying PEDOT:PSS. I want to make a PEDOT:PSS film by spin coating on the glass substrate. However, it would be throw away at only 200rpm. The substrate was precleaned by...

27 January 2021 7,862 5 View