I need to perform qRT PCRs on cDNA obtained from VERO E6 cell lines (derived from African Green monkey) and I need to choose ne (or more) housekeeping gene. I usually use B-actin and GAPDH for human samples... any advise? Thank you!!
I have a heat transfer model in the thermal bath (temperature changes in the range 23-33degC), in which a vial with PCM material is immersed (melting temperature is 28degC). During the heating...
03 January 2021 3,548 2 View
I'm pretty sure they are mostly macrophages since after a 2 hour incubation in M199 they are stuck with pseudopodia. My slides just aren't pretty enough for me. I'm using Fisherbrand Wight-Giemsa...
13 December 2020 5,475 3 View
Hi everybody, I am beginning with the inclusion of biological samples (fish larvae) in resin (i.e. Technovit 7100). I am experimenting some troubles during the cut at the microtome (I would like...
16 November 2020 2,373 3 View
Hi dear fellows, I would like to perform ChIP-PCR on the promoter sequences of genes, but I am not quite sure how to design primers for the promoter sequences. Can someone please help me? My...
20 September 2020 7,371 6 View
I want to use R to make regional climate models to analyze changes in Holdridge's life zones. I haven't used R in a long time so I'm kind of outdated and would like to read some references that...
01 July 2020 1,728 4 View
In times of pandemic, distance learning has gained strength. What can teachers do to make distance classes more attractive to students?
23 May 2020 8,148 22 View
Some journals do not accept review papers because they consider that the theoretical framework is the basis of a research, and not the research itself. Other magazines already accept and encourage...
20 May 2020 7,841 50 View
In my PhD, I want to analyse fungal communities in lake sediment cores from Siberia. One of the performed experiments will be a metabarcoding of the fungi. Therefore I am evaluating primers at the...
05 May 2020 3,920 1 View
The question aims to know what types of species are most used in ecotoxicological tests of effluents from industries in the oil sector.
26 April 2020 5,382 21 View
My hypothesis is that the functional diversity of fish (responsible variable) increases until an optimum level of a gradient of habitat structural complexity (predictor variable), but decreases...
01 April 2020 9,569 4 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View