I need to perform qRT PCRs on cDNA obtained from VERO E6 cell lines (derived from African Green monkey) and I need to choose ne (or more) housekeeping gene. I usually use B-actin and GAPDH for human samples... any advise? Thank you!!
seeking research on similarities and differences between Ubuntu and Judaism similarities between Levinas and Judaism
21 July 2024 8,545 0 View
Hi everyone, We experienced a strange pattern in some spectra coming from HRMS/MS analysis. In particular, in the MS1 spectrum a base peak at m/z=M and a smaller peak at m/z=M+5Da appear, but in...
08 July 2024 3,631 4 View
Hello I am researching this topic to obtain resources to write a paper at our facility to reinforce the need to have involvement with the local schools for a topic close to my heart nursing....
11 June 2024 6,634 5 View
If you're doing research on foresight and/or technological development and diffusion, then you might want to consider this call for abstracts. It's for a special issue of Wiley's Futures and...
02 April 2024 3,841 0 View
Etudiante en Master 1 infirmière ne pratique avancée, je réalise une revue de littérature sur cette question de départ pour en extraire une question de recherche
26 March 2024 3,081 3 View
Any information about this?
20 March 2024 1,475 0 View
within the past 5 years
06 March 2024 2,103 3 View
The regulatory institution European Food Science Authority (EFSA) publishes and maintains a list of organisms generally considered as safe for food production - The Qualified presumption of safety...
11 February 2024 8,511 0 View
I am researching information on what defines outreach.
14 December 2023 4,668 1 View
I am making an analysis to select the best model based on AIC, but one of my models gets a value of 1 in the AICcWt. I understand this basically means that this model has a power explanation of...
25 July 2023 550 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View