What's the most suitable housekeeping gene to use in RT2 qPCR array of formalin fixed bone marrow biopsies of patients with bone marrow fibrosis ?

17 May 2016 1 6K Report

• RT² Profiler PCR Array kit will be used for expression analysis of Human EMT genes, using customized plates

what is the most suitable reference gene to be used

I gave a quick check at NCBI and I couldn't find any article on qPCR using bone marrow samples. I believe you will have to assay different HKG to check which one is the best for your conditions (health vs fibrosis). Since you are using RT2 Profiler, I know that Qiagen also has a predesigned plate with 12 different endogenous control. That can help you choose the best control.

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