We subjected a bioactive glass material to antimicrobial studies and derived various zone of inhibition values. So we will like to know range of values that's good before concluding it potent as antimicrobial agent
The zone of inhibition will not all you whether your glass has promise of efficacy in clinical use. If there is a zone, it is worth investigating further, if not , it isn't (unless you have a non-leachable "kill on contact" surface). If there is a zone, the next question is duration of activity, and you need to transfer the glass to a fresh plate and measure the new zone, and keep going until there is no more zone. That will give you an idea of how long the activity lasts. Then you can decide whether to embark on further tests which might be more clinically predictive.
Thanks @ Roger. What happens when a bioactive glass placed inside E.Coli doesn't have zone of inhibition? Does it means isn't capable of inhibiting the bacteria?