How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Extraction of viral DNA from human blood?

Hi, I am looking to detect CMV DNA in immunocompromised leukemia patients followed by subsequent end-point PCR. Can anyone suggests a suitable kit for extraction of CMV DNA from human blood...

13 December 2020 4,024 5 View

Gene Cloning: What are the potential problems with doubling up with redundant WPRE and minimal CMV promoter?

From my gene of interest, Sox9, when cut into my recipient plasmid, pEZT-BM, this may include two WPRE regions and CMV promoter. This would take place if I were to cut with KpnI and NotI. As such,...

05 November 2020 8,829 1 View

CMV- PCR versus IgM ELISA for studying co-infection ?

We have been looking for the fueling effects of cytomegalovirus on HIVpositive patients. patients serum was tested for CMV by PCR and ELISA for IgM. We found discrepant results, that IgM positives...

12 October 2020 5,139 2 View

A bovine oocyte-specific transcription factor cannot overexpress in mammalian cells?

Hello all, I am trying to overexpress a oocyte-specific transcription factor in HEK293 cells. But it never worked in protein level, I cannot detect it through western blot but I do detect it...

28 September 2020 1,478 7 View

What is causing the delay in expression of EGFP in HEK293T transient transfection with pAAV plasmid?

I have an AAV2 backbone and I am trying to create viruses for my further experiments. I am making an AAV CMV EGFP virus as a negative control and it is giving me a problem. The EGFP expression in...

15 January 2020 4,057 1 View

Why when I generate a stable cell line I get a mixed phenotype even after a single cell selection?

Hi all, I have generated a few cell lines and I keep getting this mixed problem. I transfect the cells with 50-500ng of the plasmid (CMV promoter and SV40 for the selevtion marker). I add...

11 December 2019 3,522 3 View

Will a non-poxviral genome that is placed under a poxviral promoter and between two pox viral genes be expressed by CMV promoter of pCIneo plasmid?

If I construct a recombinant plasmid in the following order of genes in pCIneo plasmid: Fowlpox virus gene 1- early late promoter of poxviral origin - Non-poxviral gene - Fowlpox virus gene...

11 December 2019 393 1 View

What is the best method to detect simultaneously VZV, JEV, CMV and EBV virus by single PCR assay and gel electrophoresis?

I want to develop an assay about virus studies. So I am searching a method to detect simultaneously Venizella zooster, Japanese encephalitis, Cytomegalo virus and Epstein barr virus using only...

10 November 2019 5,435 1 View

Which mammalian promotor is suitable for weak but steady expression via transduction in mammalian cells?

I want to transduce mammalian cells with lentiviral system and later inoculate transduced cell into mice but I don't want to highly overexpress my protein after transferring the cells to mice but...

10 November 2019 7,642 3 View