20 April 2020 1 4K Report

What's the functional difference between miniCMV promoter and CMV promoter?

I didn't find any papers that explains the origin and function of the miniCMV.

Thank you very much.

More David Creation's questions See All
Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

Similar questions and discussions
Why the fibroblasts transfected with puromycin plasmids are dying after treatment of puromycin?

Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...

23 June 2024 4,902 3 View

Low-yield of Cas9 plasmid after maxi-preparation. Anyone has the same issues?

Hi~ We are making different Cas9 plasmids such as regular spCas9, base editing and prime editing under different promoter such as CMV and CAG. During selection of colonies, we always get a good...

22 May 2024 7,336 0 View

Hi. Can I use a plasmid with CMV promoter in cell-free in vitro system?

I need to express some peptides in cell-free system (fe TnT) but my plasmid has CMV promoter (no T7 or Sp6).

21 May 2024 4,964 4 View

Can I add growth medium to virus-infected cell culture plates?

Hello all. I grew some cells for virus infection. I infected the cells with CMV (Cytomegalovirus) 8 days ago. The cells have not started lysis yet, but the plate is about to get dry. I wonder if I...

08 May 2024 7,052 3 View

Is there any host plant to separate CMV and potyvirus?

I have plant sample with mixed infection of CMV and potyvirus, is there any way I can separate CMV only?

10 January 2024 3,972 1 View

What could be the reason for no expression of protein after transfection in HEK293 cells?

I have cloned my genes in a CMV vector and transfected them in HEK293 cells. After harvesting the cells, the expression level at the transcriptional level is increased compared to the control...

23 December 2023 2,507 10 View

Why CMV promoter and SV40 promoter is used used in mammalian expression vectors?

I am having a doubt regarding CMV promoter and SV40 promoter in mammalian expression vector 1. Why CMV and SV40 promoters are used in mammalian expression vectors why not others? 2. There is a CMV...

05 December 2023 1,830 3 View

CMV and EF-1alpha promoter in same plasmid?

Hi I was wondering whether CMV and EF-1alpha promoters can be put in a single plasmid? Are there any compatibility issues between the two promoters? Thank you.

31 October 2023 9,919 2 View

Why is Cytomegalovirus not routinely tested among blood donors in several countries like Nigeria?

In accordance with the research titled "Transfusion Services in Tropical Africa: Challenges and Prospects from the Nigerian Perspective" conducted by Sagir Gumel Ahmed in 2022, unlike other...

06 September 2023 4,740 1 View

Why is transfection efficiency low when using Lipofectami™ 3000 Transfection Reagent with WT Hella cells for transient transfection?

I used 0.5 ug plasmid. Cell confluency around 60%. Transfection time is 48 hour. I used empty plasmid (pCMV-3Tag-4A) and only Lipofectamine as a negative control. However, I used EGFP plasmid tag...

03 August 2023 1,883 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close