Dear Usman

It depended on your interest resolution and kind of gene.

however, I know at laest you need around 100 diverse indivisionals for Association mapping.

Also, mapping population have significant effect in mapping resolution.

I suggest read the book in below link:

https://www.springer.com/us/book/9788132223153

You can find QTL mapping in

QTL mapping in rice

and fine mapping in Article Fine Mapping and Candidate Gene Analysis of the Tiller Suppr...

Hi Babar,

Fine mapping enables you to go very close to the gene which contribute highest to the total phenotypic expression. In other words, doing fine mapping reduces the chance of recombination between the marker and gene, hence your MAS will be efficient and high chance of getting the gene through map based cloning (that's the reason why you have been or you are stared to do fine mapping)

When you have mapped a certain genomic region/s if its really big there would be high chance of recombination and hence, lose marker trait association. So, we need to go for fine mapping to reduce the chance of recombination and hence high marker trait association and efficient MAS.

To do so, its always better to have large population derived from the recombinants of the respective locus. It depends on size of the locus you are targetting. Smaller the size larger the population required.

You may follow one of my research articles for further understanding, Article Identification and fine mapping of a new gene, BPH31 conferr...

Thank you! Hope information will be useful.

Thanks Sir, Its really very valuable comment from your side. I'll turn to you if I need more guidance in future. Thanks

Dear Mostafa Haghpanah

Do you have this book?

Marker-Assisted Plant Breeding: Principles and Practices

Authors: Singh, B.D., Singh, A.K.

The textbook entitled" Marker-Assisted Plant Breeding: Principles and Practices. Authored By: Singh, B.D., Singh, A.K. could be purchased from Springer. You can purchase from there.

QTL mapping is a genome-wide inference of the relationship between genotype at various genomic locations and phenotype for a set of quantitative traits in terms of the number, genomic positions, effects and interaction of quantitative trait loci (QTL). QTL mapping: NILs, RILs, Chromosome segment substitution lines (CSSLs), Immortalized F2 populations etc.

Fine mapping is the analysis of very large populations using a sufficiently large number of markers to identify markers located very close to a gene known to be linked to a marker. The mapping populations for fine mapping: RILs, Chromosome segment substitution lines (CSSLs), Immortalized F2 populations, MAGIC populations etc.

The papers attached would also be beneficial for you to conduct fine mapping experiments in crop plants.

Is there a difference between QTL mapping and gene mapping?

Hello,

Is there any difference between fine mapping and meta QTL analysis. Fine mapping is reducing the recombination between trait and marker. region b/w markers will be reanalyzed. In metaQTL: region of QTL having high LOD score will be re analyzed.