Mouse tissue sample
I am seeking advice on the matrix and solvent conditions.
29 May 2024 2,360 0 View
I found it very difficult to define it precisely. Microspore and megaspore of ferns or seed plants are sexual spore? Because the fertilization happened for a long time before the formation of...
27 April 2024 3,077 0 View
Hi all, I'm Dabin Xie from Zhincai Tech, a small research-driving company in China. I'm currently trying to modify glass with a single layer of silane whether hydrophobic or hydrophilic, like...
08 April 2024 2,628 4 View
How to solve this problem when Aspen economic evaluation was performed? It said "Temperature difference is insufficient to perform the heat transfer."
06 April 2024 7,291 3 View
If I want numerical simulation software to simulate superconducting magnetic shielding, which software is better for simulating superconducting magnetic fields?
25 March 2024 5,589 1 View
I've encountered an issue following an LR reaction intended to construct a gene within a pENTR-3C (KanR) vector and combine it with a pCDH (AmpR) vector. Despite expectations, the protein was not...
14 February 2024 3,502 0 View
I tried to make a moive of protein structure in PyMOL. However, when I stored the movie for future use, the PyMOL reminded that the encoders were not installed. How to install these encoders in...
29 October 2023 5,129 2 View
I have performed the 3C-qPCR assay and designed the primers of interested region, but I do not have BAC clone as control. How should I normalize the prime efficiency and background noise?
16 October 2023 4,387 0 View
In the XRD experimental analysis of polycrystalline TiN films, are TiN (111) and TiN (222) different crystal planes? Or are they the result of different diffraction levels of the same crystal...
26 September 2023 3,580 5 View
If a protein was designed as a closed cyclic protein (N-and C-terminus closed after reaction), can PyMOL show this structure? Because in PyMOL, the sequence is always linear, so how the closed...
17 September 2023 2,886 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
