I found AAV6 can infect lung, but its efficiency is not so good , improvements are still needed. Does anyone have any idea about the better serotypes than AAV9 for mouse lung infection, maybe capsid mutation or peptide display can screen out some candidate.
I wonder if AAV engineered with SARSCOV2 RBD surface protein will increase lung infectivity? Best
As far as I know, AAV4 & 6 are the most efficient natural serotypes regarding the transduction of airway epithelial cells and alveolar cells. They can be applied intranasally. Efficient application, however, requires some experience. We've tried both (AAV4&6) and only achieved moderate results. With regard to pulmonary (microvascular) endothelial cells, there are AAV capsid mutants that work quite well upon intravenous administration. Our AAV2-ESGHGYF is one example (PMID: 27018516).
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