What's a good buffer for extracting bound sirius red from tissue?

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Gabriela Ciapetti

Dear Heins,

to quantitate collagen in cell cultures, 0.1 M NaOH was used to extract picrosirius red to be read at 540 nm in a spectrophotometer. I did not test this staining on collagen-rich tissue, but, according to Sircol instructions, 1N NaOH has to be used. I think you have to test your experimental system to find what is the best. Good work!

Merlin Walter

Agree with above, I have always used 1M NaOH for this.

Heinz Scheuenstuhl

Cool. Thank you both!