What would be the possible reason of getting nonspecific band using SCAR markers?

More Milad Rashidifard's questions See All

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to achieve the sensitivity diagram of a sensor in CST?

I wanna design a sensor in CST 2021 for the first time and I don't know how to achieve the sensitivity diagram of the sensor in CST. Could you plz help me to do that? I appreciate your response...

26 January 2021 6,864 1 View

What kinds of potential genes could be expressed in frogs exposed to chytrid fungus in different environmental conditions ?

I need an applicable molecular biomarker like expressing the genes in frogs who expose to chytrid fungus in different environmental stressors. Indeed, what kinds of genes I should notice in frog's...

25 January 2021 7,718 2 View

How to select the most non-correlated samples within a dateset?

Dear researchers How to select the most non-correlated samples within a dateset? Considering that the samples are time-series of length l within m different time-series. To more clarify the...

13 January 2021 741 6 View

DSC test- Glass transition temperature of the electrospun PA66 nanofibers?

Hello Everyone, I did several DSC tests on the electrospun PA66 nanofibers to obtain the glass transition temperature. According to the material datasheet, the Tg point of PA66, as cast, is around...

13 December 2020 1,506 2 View

How to create a sag and swell detection in the DVR applications?

I have created a three-phase cascaded h-bridge inverter at Simulink with the SHE-PWM technique, and I want to test it in the DVR applications. How can I design a controller for that in the DVR...

13 December 2020 735 3 View

Degenerated semiconductor (Burstein Moss effect) with unchanged band-gap?

Is it possible to produce a degenerate semiconductor using a dopant agent that results in increasing the electrical conductivity, but the optical band-gap remains unchanged? In such a situation...

11 December 2020 8,338 13 View

Which statistic software can give me a relationship for multivariate function?

Hi friend I have some data and they influence on each others and I draw their graph in excel but excel can't give me a relationship for multivariate function. the regression of these data in excel...

29 November 2020 9,371 9 View

A paper may be rejected because of several reasons?

In my case, I have received rejection after seven months from submission to journal without reason or editor comments (decline). Moreover, without being reviewed by an expert scientist reviewer,...

29 October 2020 5,437 4 View

Do scalar-tensor-vector gravity theories violate the Bekenstein-Hawking area law to compute the entropy of black holes?

I am looking for the thermodynamics of the cosmological black hole in scalar-tensor-vector gravity theories. I know how should I write and find the thermodynamical quantities and equations for...

26 September 2020 6,383 3 View

Can someone explain how to carry out DNA fingerprinting in crop plant without reference genome and no locus information?

Is it possible to develop DNA fingerprinting for varietal identification of crop plants without reference genome and no locus information except the availability of set of DNA markers.

25 January 2021 6,826 4 View

Why my ladder bands are not properly stained?

Hi. I am doing an agarose gel electrophoresis. After running PCR samples in a 2% agarose gel at 90V x 45' I obtained the results in the image. The ladder bands are not properly stained. For...

14 January 2021 2,316 4 View

How to estimate the effect of nematodes on some varieties of wheat?what are the correlation possible to do ?

Hi , I carried out a test on the behavior of some varieties of durum wheat with respect to the CCNs, two successive years, in pots, and I inoculated with the same number of cysts (Pi), at the end...

05 December 2020 7,187 3 View

Trouble with a promoter cloning.how can i resolve it?

Hi everyone .. I have great difficulty in cloning a promoter of a stem cell marker in an expression vector. the region is high GC percentage and in fact in the pcr, I had to use both adequate...

08 November 2020 8,763 3 View

How to fetch data of a .sslist file?

We are using Mauve software for alignment of genomes of nematodes having genomic size approximately 100 Mb. One of the output file generated using this software has extension .sslist file. Unable...

24 September 2020 7,520 1 View

Is there any necessary to have negative controls when FACS detecting neural stem cell markers?

Dear fellow researchers, I've been using flow cytometry to test our iPSC-derived NSCs for biomarkers, such as SOX2, Nestin, Musashi-1 and vimentin. I'm wondering what would be the best...

05 August 2020 3,406 4 View

Which genetic marker (nuclear DNA) should I use for phylogenetic analysis of fish?

I am studying two types of Ophidiid fishes having features morphologically similar and would like to genetically classify them. I have already investigated COI region of mtDNA, but the genetic...

14 July 2020 7,649 7 View

Can someone tell why there is empty result file in Structure?

Hi everyone I am running Structure version 2.3.4 programme smoothly.But now I am facing problem that in Result folder I am getting only one file although I am setting k=2 to k=10 and iterations...

27 June 2020 2,330 3 View

Could someone recommend me a journal for the publication of a short note discussing the validity of some parasitic nematode species?

These species were described in a PhD thesis and all of them but one remain unpublished.

06 June 2020 4,786 3 View

What is the difference betweeen mesenchymal markers and stem cell markers in CTCs?

For example CD44 is a stem cell marker, whereas Vimentin is an EMT marker. Mesenchymal cells are called MSCs (Mesenchymal Stem Cells), then why are there different markers for stem cells and...

01 June 2020 2,637 5 View