Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

Why do my cells die during transfection with JetPei?

Hello everyone, two months ago I did a transfection of HEKs cells to insert a miR using the JetPei reagent. On that occasion, the transfection was highly efficient and the cells were in perfect...

03 February 2021 1,296 6 View

How to obtain the plot of frequency shift w.r.t. pressure in a micro resonator (cc beam) based MEMS pressure sensor in COMSOL?

I need to know how to plot the impact of pressure (0 - 100 kPa) (which is applied on the back side of diaphragm) on the frequency shift caused in the cc beam supported on the diaphragm with...

01 February 2021 5,805 17 View

Inexpensive eye tracking hardware + software solution?

Hello, I am starting a project that will require an eye tracking device. I've seen eye tracking hardware + software solutions that are $10,000+ so I am hoping to find one that is significantly...

18 January 2021 8,121 12 View

What is the simplest way to implement data encryption for Arduino project that communicate through Bluetooth ?

I have developed a project based on Arduino that communicates through Bluetooth with Android application, I want to enhance its privacy through encryption so that data should be prevented up to...

07 January 2021 7,933 5 View

How to configure wifi password and SSID on NodeMCU ESP8266 through wifi direct instead on hardcoded credentials?

While working on IoT projects I mostly use NodeMCU ESP8266 firmware for IoT Implementation. For coding, I use Arduino IDE in which for connectivity with wifi router we define hardcoded variable...

07 January 2021 6,688 3 View

What materials besides gold can be infused into window panes?

Hello, Driving around, I have noticed a lot of office buildings where the glass panes have been infused with gold. The thin layer of gold reflects the sun and keeps the office at a comfortable,...

06 January 2021 976 4 View

What's the reasonable loading control of nuclear protein in acute injury?

What's the reasonable loading control of nuclear protein in acute injury? Is HDAC1 or Histone H3 a resonable loading control of nuclear protein in acute injury model? How to explain total my total...

06 January 2021 1,607 2 View

Why the storage moduli E' decrease with highr fiber volume?

Hi guys, I am facing problems to interpret a DMA results. Material: Composite Bisphenol A + curaua fiber What is being tested: the influence of fiber volume on the composites properties...

12 February 2021 174 7 View

What can I do to keep magnetic beads suspended in solution within a syringe?

I'm looking for ways to prevent the settling of magnetic beads in solution. I have been using a syringe pump to inject a suspension of magnetic beads in PBS into a microfluidic device, and so far...

09 February 2021 6,206 2 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

Is it better to push or pull liquid through microfluidics with a syringe pump?

I have a PDMS microfluidic device that I want to perfuse cells through (there are also adherent cells in the channels). Is there any difference if I use a syringe pump to push the cells through...

30 January 2021 4,092 3 View

Is there any software to generate pdb of G quadruplex?

I have a DNA sequence which has predictability to form G quadruplex . I need a software to generate a pdb of it which will form G quadruplex. I am using open Babel but I am not able to set the...

29 January 2021 6,190 4 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View

How can I measure speed of integration of an acquisition with secondary data?

I am currently faced with the challenge to measure integration speed of an acquisition or a merger with secondary data. Usually companies do not communicate once a target has been integrated...

27 January 2021 9,741 11 View

For DNA purification, what is the difference between purifying magnetic beads and column?

Hi! I noticed that mostly the single cell assay use AMPure beads (magnetic) instead of purifying column. But both function through a DNA affinity coating, just on surface in beads and on membrane...

27 January 2021 9,043 5 View

Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

How did teaching writing gain its ground in the course of time ?

Since the decline of audiolingualism, there was bias towards speech in language teaching for communicative ends. Though, the stress on speaking rather than writing produces fluent but inaccurate...

12 January 2021 517 4 View