Which factor would play the major role if culture of E.coli is grown in ready made media (LB) and media made of raw materials (yeast extract and dextrose)?
There are different nanostructures of silicon like nanotubes, QDs and silicene. I need to know what are the 'green' or other effective methods for preparing them..please share the procedures of...
10 January 2021 3,418 1 View
technology relevant methods or processes
02 January 2021 6,632 2 View
Adsorbed water and gases can affect the weight of the porous carbon. Some times 6-7% weight loss can occur during the degassing of the sample. Will this weight loss affect the calculation of C,...
08 November 2020 5,399 3 View
Sir, How I Can filter the only RNA as well as DNA separately from the whole genome after lysing using a membrane, and which membrane filter is suitable for the same.
14 September 2020 601 1 View
I have written code for a for a Heat Exchange Reformer in Aspen Custom Modeler, but it cannot run. The program describes a shell and tube heat exchanger with steam methane reforming reactions...
07 September 2020 2,024 9 View
Hi Everyone, Entecavir 0.5 mg is usually given for patients with chronic hepatitis B to keep the virus under control and as well Vaccine is available for restricting the virus. If Entecavir 0.5...
22 June 2020 5,661 3 View
In information theory, the entropy of a variable is the amount of information contained in the variable. One way to understand the concept of the amount of information is to tie it to how...
01 May 2020 6,703 6 View
For academic purpose, free and open source simulation tool for internet of things
09 April 2020 7,137 11 View
I have got a publication in which the authors prepared BaFe12O19/CoFe2O4. But the amount / concentration used for preparing the metal nitrate solutions were not mentioned in the publication...
15 January 2020 3,563 2 View
I already found the human chromosome DNA sequence from the ensemble browser .But the problem is in that sequence it shows some N terms are coming top , bottom and in the middle .Without N terms...
12 December 2019 7,218 4 View
Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...
23 February 2021 1,908 2 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Hello, I am looking for alternatives or cheaper materials to isolate out MS2 from E. coli following lysis via chloroform. Some literature I have reviewed uses a .45 um nanopore filter following...
02 February 2021 807 3 View
I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...
25 January 2021 5,736 3 View
What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?
24 January 2021 9,987 3 View
I'm looking for an open repository or culture collection to deposit some genetically engineered E. coli strains (from the papers below). The primary goal is to preserve those strains for the long...
11 January 2021 4,398 1 View
Hi. I need help to measure trypsin inhibitor activity in fermented soy and fermented oat samples. The problem is my samples were mixed with water with ratio 1:3 and 1:2 respectively, for...
13 December 2020 6,882 3 View
If I have a gene carried on a plasmid and the plasmid is transformed into two strains of different bacterial species (Salmonella and e. coli). If I want to do RT-qPCR to quantify the expression of...
03 December 2020 8,051 5 View
For reference: I am transforming a newly assembled plasmid what happened: I accidentally incubated my electroporated e.coli cells on the shaker at 30C for 1 hr rather than 37C and did not...
02 December 2020 7,133 5 View