Which factor would play the major role if culture of E.coli is grown in ready made media (LB) and media made of raw materials (yeast extract and dextrose)?
Hello connection While performing potency test by using BD FACS instrument some time we few population inside the gate few outside of gate and some time I got population outside of the gate I am...
17 March 2024 3,511 4 View
effect of childhood trauma on mental health
12 September 2023 5,093 2 View
Kindly provide the details
30 August 2023 4,884 1 View
I am referring Theoretical studies on electrochemistry of p-aminophenol paper for calculating the theoretical redox potential using the described in this paper. I reproduce the DFT calculations...
18 August 2023 3,573 1 View
I encountered the calculations in the research article titled "Theoretical studies on electrochemistry of p-aminophenol ." However, I'm having difficulty comprehending the procedure used to...
13 August 2023 1,639 1 View
I have a query is that after crystallization peak point some of other noise type peaks occur in DSC scan for (Se78-xTe20Sn2Znx) x=2 and x=6 ,this peak shown the material property or due to...
05 August 2023 8,945 1 View
I am trying to optimize the geometry of a reactant complex obtained from the IRC. The geometry of the product complex is getting optimized, but in the reactant complex, the maximum force is not...
23 May 2023 8,098 3 View
Hello everyone, In my case, i ligated my insert (804 bp) into pET30a expression vector (5422bp) 3:1 ratio containing 20 ul reaction by using T4 DNA ligase and incubated at 24 degree centigrade...
16 May 2023 884 3 View
Hello everyone, I have one doubt regarding cloning ? I am using pET30a (+) vector for cloning (5422bp) and Insert size 804bp. I tried all the ligation reaction ratio lilke 1:3, 1:5, 1;7 (100ng...
26 March 2023 7,023 6 View
Some journal reviewers prefer that the rate performance of batteries be expressed in C rate rather than A/g. However, since battery capacities vary across different chemistries, wouldn't A/g be a...
20 February 2023 973 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
As PhD student specialized as food and industrial microbiology need to further research on bacteriocin and fermented food.
16 July 2024 7,394 1 View
any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...
13 July 2024 2,255 3 View
What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...
13 July 2024 2,431 2 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
How we should screen out the solid material left after 90 days of fermentation in making of garbage enzyme. Is there any standardized process.
17 June 2024 4,840 3 View
Last week, I transformed home-prepared E. coli TOP10 cells. When checking the colonies, I noticed that some of them have yellow color. They did not grow on the randomly, but dependently on the...
16 June 2024 3,272 3 View
Hello! I am conducting fermentation experiments using the BioFlow 3000 bioreactor. The manual says I should use the BioCommand Lite Software to control the bioreactor and collect data. However, I...
02 June 2024 6,505 5 View
i have a species trichoderma harzianum. It is development in PDA media normally. But it is producing yellow pigment on rice broken at fermentation.
31 May 2024 9,260 0 View