I am working on a new project involving the vector of malaria and I would like to know, among the various methods, which is the best for conserving DNA. Some references would be useful.
Thanks in advance.
In the appropriate buffer you can store your DNA samples at -20 or -80°C. Making small aliquots is also very important. Chek this link:
http://www.ogt.com/resources/literature/403_dna_storage_and_quality
regards
You can simply store them at -20. If you have to use each individually, put each of them in separate appendorf tubes else pool them and secure at -20.
I agree that storage at -20C is probably the most safest way to preserve DNA. Repeated metling/freezing cycles usually do not influence downstream analysis (PCR, restriction enzyme digestion, Southern hybriidsation,.. etc).
In the case of large number of samples and limited capacity of freezers the DNA can be maintained for several months in a fridge (+4C). Such storage require following conditions to be fulfiled: DNA should be free of proteinous contaminants (nucleases). It should be dissolved in a stabilizing TE buffer (10 mM Tris-HCl pH-8.0 , 1 mM EDTA).
More in a Holy bible of molecular biology:: http://nhjy.hzau.edu.cn/kech/jycz/jczs/ml-introduction/Content.pdf
Thank you Ali Mahmoudpour, Anushrita Singh, Matthias R. Schaefer and Ales Kovarik, the observations were useful. And Juan Ospina, the link provided was very useful too, thank you.
I wanted to clarify that I have not done the extraction yet. I need to save the samples and then extract the total DNA. And in fact I was already wondering if I would need some solution to preserve the DNA besides storing it at low temperatures.
While originally marketed for RNA preservation in tissue, RNAlater from ThermoFisher works well for preserving the DNA the specimen, tissue, or in your case mosquito and can be kept at room temp. RNAlater minimizes the need to immediately process or freeze the samples in liquid N2.
https://www.thermofisher.com/order/catalog/product/AM7021?ICID=search-product https://www.thermofisher.com/us/en/home/references/protocols/nucleic-acid-purification-and-analysis/rna-protocol/genomic-dna-preparation-from-rnalater-preserved-tissues.html
Another option could be lyophilization apart from storing in -20 degree freezer.
The STAR buffer from Roche is possible to use and is made to preserve dna at room temperature when shipping stool samples. I have used it to preserve bee gut samples and tested it before and after storage....no loss of bacterial dna. Mixing 1/3 with sample and buffer.
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