What would be the best transport and culture media to isolate bacteria from endodontic infections?

More Aymen S. Yassin's questions See All

Copyright if i publish on researchgate?

If I publish a preprint on ResearchGate that was accepted on SSRN, for those who find it on ResearchGate, does the non-commercial license from SSRN apply, or will it just be CC-BY as offered on...

02 May 2024 6,999 4 View

Is publishing new research the best way to increase RG scores?

Is publishing new research the best way to increase RG scores or there are more effective ways to get the scores?

11 April 2024 1,810 1 View

Opening and emptying the content of fluconazole capsule in a glass of water does affects it's absorption and afficacy?

We all know that the absorption of fluconazole is unaffected by food or gastric PH....... So, what's the effect of emptying the content of fluconazole capsule in glass of water on drug afficacy???

12 November 2023 1,328 0 View

What to do to reduce non-specific hybridization & primer dimer formation particularly?

I have tried lowering the Tm, worked on human DNA but not on mice DNA. Tried optimizing denaturation temperatures too. Any suggestions?

22 September 2023 2,405 4 View

Immerging of hydrophobic fiber before mixing with the cement mortar's dry components has any effect on the hydration of cement mortar?

does the hydrophobic fiber, with no immersion in water, absorb a small amount of cement's mortar mixing water?

30 June 2023 5,368 1 View

What is the known europeans standards for the proportions of main components (cement+sand+water) to prepare a cement mortar?

is the following mixture respect the europeans standards? cement => 1 part sand => 3 parts water => 1/2 part

30 June 2023 8,350 4 View

What is an efficient method for accurately determining wear volume of flat plate wear scar (pin-on- plate tribometer)?

03 June 2023 2,598 0 View

What is the interpretation of 2theta=26.65° in a XRD pattern of a natural tree bark powder?

Which component of the tree bark is characterized by this diffraction angle?

31 May 2023 9,827 3 View

What is the best way to spot phosvitin stability, extracted from egg yolk, after heat treatment?

For protein extraction i found a protocol that separate phosvitin from egg yolk using ultrafiltration trough 10 MWCO then uses the filtrate. But phosvitin molecular weight is 35 KDa. So how it is...

16 April 2023 4,309 2 View

Convergence criterion comsol???

Hi all.I am working on a flow with heating in a channel and I use Comsol when I use a small mesh The solution works fine when I increase the mesh the calculation is diverged.does anyone can help...

05 February 2023 4,772 3 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

What are the best (less toxic to human) methods of microbial lab fumigation?

We are planning to establish a microbial laboratory in our research center and are actively seeking the best disinfection methods. If anyone has any recommendations, please let us know. Thank you.

04 August 2024 1,973 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How does social media influence people's dental habits and interest in aesthetic dental treatments?

Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...

01 August 2024 9,349 2 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Amit Agrawal

I guess 'stab culture' would be best way to transport. Media am not sure, but blood agar would work well.

Eldho Jijy Varghese

Best culture media will be thioglycolate broth and agar media, best storage media will be anaerobic jar..you can check my article based on novel antibacterial dental composite based on nano ceria in which I have used antibacterial analysis