Does anyone know why I have got multiple unclear bands image of a western blot?

I'm doing western blot to detect LRRC8A protein which is extracted from colon cancer cell HCT116, unfortunately, I could not find a good result. Does anyone have an idea or advice?

21 November 2019 2,106 3 View

I want to simulate concrete column wrapped with CFRP with ANSYS finite element ...can any one please provide me with a reference or video could help.?

This is my E-mail : [email protected]

18 June 2018 8,662 4 View

Can an ELISA assay for glucocorticoids (GCs) measurements be used in assaying GCs in tissue lysates?

I would like to measure GCs in tissue samples and I have tissue lysates. Is it feasible to use an ELISA-based assay or is there a better alternative? Thanks in advance!

04 April 2018 5,578 1 View

How can I get the values of tb,td(beam and diffuse optical depths)ab,and ad(beam and diffuse air mass exponents) according to latitude and longitude ?

03 August 2017 5,307 4 View

Is SILAR technique can be useful for preparation of 2D monolayers? e.g. boron nitride?

Is SILAR technique can be useful for preparation of 2D monolayers e.g. boron nitride?

14 March 2017 5,421 1 View

Is there an exact method to sub-fraction plants extract ? Does it differ from one plant to another?

The plants are from lamiaeae family

15 May 2015 9,351 5 View

Is there a protocol to sub-fraction the extracted materials from a plant?

does this protocol differ from plant to another

14 May 2015 8,454 7 View

What is the main risk of password authentication in cloud computing?

This field considers the heart of security world.

07 May 2014 8,414 2 View

Dear Friends, do you know where can I find solar flux of specific location for last 4 years in W/m^2. latest available data just till 2005?

The required data should be hourly if that possible or at least daily. the data that I need for Istanbul city in Turkey. I try to find some data but what I found was for 2005 and 2004 and so one...

01 January 1970 1,559 5 View

Using Virtual Reality in Education - Teaching Students in a classroom

What does the research say about Virtual Reality in Classrooms? What are the kinds of tools could be used for this purpose?

01 January 1970 795 6 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View