We bought DMEM with high glucose, but someone told us that it is toxic to cells! If we reduce the concentration of media does it work?
It depends what research you are doing and what you are looking at. High glucose concentration in the culture medium induces HepG2 cell proliferation.
You cannot dilute the culture medium (with water) as all the other components would then be at the wrong concentration. You could possibly dilute with low glucose DMEM, but the medium would obviously still have higher glucose than normal.
To be on the safe side, why don't you give your high glucose DMEM to a group that uses it, and ask them to buy you some normal DMEM in return.
Premixed DMEM can be purchased in three formulations with respect to glucose concentrations: high (4.5g/L), low (1g/L) and no glucose.
If you do not want to buy low glucose version (assuming that is the target concentration for all of your future expts. and you already have a large stock of high glucose medium, which you do not wish to waste), buy without glucose and mix it with what you already have in proportions to get the desired concentration of glucose.
In our hands, high glucose DMEM does not seem to be toxic to hepatoma cells.
We grow our HepG2 cells routinely in DMEM with high glucose without any toxic effects.
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