Hi all, I am calculating amount of (nh4)2so4 I will add in protein sample.I will increase concentration by 10% every time until it reaches 90%. Now the starting concentration is 99.5% provided by the manufacturer, I will need 50% from 99.5% for 3mL protein sample. Next stage is to increase by 10% and the volume of sample will be 2.5mL. But this time what will be the initial concentration of (nh4)2so4? will it be 99.5% or 50%? I will take 10% from the manufacturer's. The desired concentration will be 60%.
I hope I dont sound confusing.
Tamjeed Saleh
http://www.sigmaaldrich.com/chemistry/stockroom-reagents/learning-center/technical-library/solution-dilution-calculator.html
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