Tamjeed Saleh

4 Questions 45 Answers 0 Followers

Questions related from Tamjeed Saleh

What primer concentrations would one use to introduce 2-3 amino acid substitutions in one site directed mutagenesis experiment?

Are there any specific changes to the protocol that are necessary for success? How many colonies need to be sequenced on average?

10 October 2014 6,294 3 View

What software do you regularly use for Protein NMR analysis?

I have been using NMRView. I wanted to know if any users here are using CCPNMR and what your thoughts are.

05 May 2014 7,270 2 View

What is your experience with split inteins?

Have you ever tried it in your lab/hands? What have you found? What works best?

01 January 2014 7,681 7 View

Is there a resin that can be used instead of P11 resin?

I have to purify a protein that involves the purification steps similar to the one in the link http://enzyme.ucd.ie/main/index.php/PKA_purification However we do not have P11 resin and it appears...

01 January 2014 1,448 4 View