Can i use Lactose instead of IPTG for M9 protein expression?

Hello all, Is it possible to use lactose instead of IPTG for M9 protein expression? Glucose is traditionally used as the carbon source for labelling proteins and we used 2% final in the media....

14 October 2020 3,584 4 View

Which protons are exchangable in a protein expression in 100% D2O minimal media?

Hello, I have purified a protein in minial media with 100%D2O, what i wish to know is if this is completely deuterated? I have sent this for mass spec analysis maldi with the matrix acidified...

18 February 2020 8,370 3 View

Can you suggest any study to affirm binding of small peptides interaction with protein which require low concentration of the ligand?

Hello, I want to ask you all for suggestion of any assay of equipment which would require low concentration of ligand to study interaction with protein(K_on and K_off). I understand that ITC...

09 April 2019 4,352 7 View

How does the dealloying process carried out, in order to produce nanoporous structure?

I would like to know more about the de-alloying process and how it is practically carried out. Is it applicable for all alloys? What are all the limitations?

25 January 2018 530 4 View

How does the microstructure of a sintered alloy vary from conventionally cast alloy of same composition?

I would like to understand the key differences between the microstructure of a cast alloy and sintered alloy, especially in terms of the formation and distribution of the intermetallic phases....

03 January 2018 4,475 4 View

Is it possible to proceed further in DICTRA calculations, if we don't have a certain phase in Mobility database?

I am doing the DICTRA simulation for AL12MG17_A12 phase. But this phase is not available in any of the mobility databases. Can anyone suggest me where I can find the mobility data for this phase,...

24 November 2015 4,796 3 View

What is the concentration of dichlorofluorecein (CM-H2DCF-DA) used in neonatal rat cardiomyocytes for flow cytometry experiments?

Can anyone suggest me please?

26 August 2014 5,154 3 View

Which type of cell line is suitable for astrocytoma related research in neurooncology?

C6 cell line is suitable for the astrocytoma related research in neurooncology.

06 January 2014 5,367 3 View

Surface diffusion during sintering

The diffusion of atoms during sintering has different mechanisms (surface, lattice, evaporation and condensation, grain boundary etc..). I would like to know, if there is some inherent oxide layer...

01 January 1970 4,546 4 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View