Can i use Lactose instead of IPTG for M9 protein expression?

Hello all, Is it possible to use lactose instead of IPTG for M9 protein expression? Glucose is traditionally used as the carbon source for labelling proteins and we used 2% final in the media....

14 October 2020 3,584 4 View

Which protons are exchangable in a protein expression in 100% D2O minimal media?

Hello, I have purified a protein in minial media with 100%D2O, what i wish to know is if this is completely deuterated? I have sent this for mass spec analysis maldi with the matrix acidified...

18 February 2020 8,370 3 View

Can you suggest any study to affirm binding of small peptides interaction with protein which require low concentration of the ligand?

Hello, I want to ask you all for suggestion of any assay of equipment which would require low concentration of ligand to study interaction with protein(K_on and K_off). I understand that ITC...

09 April 2019 4,352 7 View

How does the microstructure of a sintered alloy vary from conventionally cast alloy of same composition?

I would like to understand the key differences between the microstructure of a cast alloy and sintered alloy, especially in terms of the formation and distribution of the intermetallic phases....

03 January 2018 4,475 4 View

Is it possible to proceed further in DICTRA calculations, if we don't have a certain phase in Mobility database?

I am doing the DICTRA simulation for AL12MG17_A12 phase. But this phase is not available in any of the mobility databases. Can anyone suggest me where I can find the mobility data for this phase,...

24 November 2015 4,796 3 View

What is the concentration of dichlorofluorecein (CM-H2DCF-DA) used in neonatal rat cardiomyocytes for flow cytometry experiments?

Can anyone suggest me please?

26 August 2014 5,154 3 View

Which type of cell line is suitable for astrocytoma related research in neurooncology?

C6 cell line is suitable for the astrocytoma related research in neurooncology.

06 January 2014 5,367 3 View

Surface diffusion during sintering

The diffusion of atoms during sintering has different mechanisms (surface, lattice, evaporation and condensation, grain boundary etc..). I would like to know, if there is some inherent oxide layer...

01 January 1970 4,546 4 View

What will be the influence of feedstock powder on the microstructure?

Dear Prof Raabe, In this project, are you planning to use elemental powder to produce alloys, or will gas atomized alloy powders be used?. In case of gas atomized powder, will there be any...

01 January 1970 4,145 2 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

How to determine solutionizing time for cast Al alloys?

For wrought alloys soaking time at solutionizing temperature is determined by thickness .Do we have such standards for cast Al alloys

23 February 2021 8,839 3 View

Which thermal conductivity test recommended for Ag-Cu nanofluid?

I have prepared Ag-Cu alloy with polymer coating upon it.... how can I measure thermal conductivity of it ?

22 February 2021 5,915 3 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

What are damage parameters for SMA while modelling on ABAQUS 2020?

I have given Elastic and Superelastic material properties, but I think we need to provide the damage parameters also. Please help me here.

16 February 2021 8,694 1 View

Are there alternatives to the Devarda alloy when estimating nitrogen?

Are there alternatives to the Devarda alloy when estimating nitrogen? devarda alloy is reducing the nitrate to ammonium

15 February 2021 8,838 3 View

Passivation of chromium cast Iron in high temperature?

14 February 2021 2,157 3 View

How can we quantify martensite in Ferrous and Titanium alloys?

How can we quantify metastable phases like martensite in Ferrous and Titanium alloys, in relation to their stable counterpart For example in EBSD, XRD, Optical? Eg, Martensite and Ferrite maps.

09 February 2021 7,572 3 View

About forging of Mo Alloys ?

Hello dear Academicians, I am working on the forging of Mo alloys, but I wanted to consult you on an issue I could not solve. Mo is easily oxidized during forging and MoO3 is formed. Is it...

09 February 2021 1,989 3 View