Hi,
I am working with a protein stock concentration 9mg/ml which precipitates in the drop as soon as i mix the reservoir, although, it is not a repeated event because most of the crystals solved in PDB of this protein were solved with the same condition. I am curious because the screening was done with 20mg/ml stocka nd i set up drops of 1:1, 2:1 and 1:2 but, all of them show only precipitates. So, i am curious if there is a workaround this phenomenon when setting up expansion plates?
Hi Subhash,
According to your description, I think you can try to remove the flexible region. Moreover, you can try the hanging drop method, the nucleation rate of hanging drop method is slower than that of sitting drop method, but the crystals formed are larger and more regular, which is more conducive to diffraction. If you want to speed up nucleation, try the 18℃ condition.
Good luck!
Roger Davis : I have set up plates by hanging drop method but, the precipitation is instant. But, this was not observed in the screens
- Badges
- Science topic
- Similar topics
- Correction