Does using taxol to stabilize tubulin polymerization to study microtubule-binding proteins either by EM or NMR acceptable? Does taxol influence a different organization of microtubule assembly invitro? Is the assembly different if it is done at close to native conditions like using GTP in a common buffer at low temperature(4OC) and high-speed spin?
Prashant Singh
Yes, you can use taxol. You can also stabilize using gmpcpp. Here are some protocols that you can try:
GMPCPP stabilized microtubule: https://puresoluble.com/generating-gmpcpp-stabilized-microtubules/
Taxol stabilized microtubule: https://puresoluble.com/generating-taxol-stabilized-microtubules/
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Filing
More Subhash Narasimhan's questions See All
Similar questions and discussions