What will be a suitable mobile phase for the HPLC analysis of monolaurin? Also, will a refractive index detector be okay?

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Markus Christ

Article Determination of mono-, di-, and trilaurin in modified cocon...

Omar Gonzalez-Ortega

It depends on the solubility of the molecule in water, methanol, or acetonitrile; which are the common constituents of mobile phases In reversed phase cromatography, which I assume you intend to use. If the molecule has a reported refraction index then the detector you suggest might work.

Bruce Neagle

If an RI detector is used, the mobile phase mode must be isocratic (50/50 ACN/water?). The sample would also have to be a 'neat' sample since the RI detector is very insensitive.