What volume and concentration of Trichloroacetic acid (TCA) can be used for deproteinization of the sample containing target Exopolysaccharide?

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Jyoti Kaushik

Hello,

U can check a deproteinization kit provided by biovision company (Catalog #K808-200).

Its a very good method to remove proteins from the plant extracts and its efficency is also very good.

Pervaiz Ali

Thank you jyoti Kaushik for you valuable input.

Ashwini Kumar Dubey

Dear Ali,

You can use ice cold 12% w/v (final concentration) of TCA to deproteinise the cell free supernatant. My this article may help you,

https://www.researchgate.net/publication/285672507_Structural_characterization_and_functional_evaluation_of_an_exopolysaccharide_produced_by_Weissella_confusa_AJ53_an_isolate_from_fermented_Uttapam_batter_supplemented_with_Piper_betle_L_leaves

Regards,

Ashwini Dubey

Article Structural characterization and functional evaluation of an ...

Thank you dear Ashwini. I will go through the paper.