I am working on the production of exoploysacchardes from Psychrophilic bacteria. I need deproteinization of the sample before precipitating EPS. Any alternative method will be appreciated.
Hello,
U can check a deproteinization kit provided by biovision company (Catalog #K808-200).
Its a very good method to remove proteins from the plant extracts and its efficency is also very good.
Dear Ali,
You can use ice cold 12% w/v (final concentration) of TCA to deproteinise the cell free supernatant. My this article may help you,
https://www.researchgate.net/publication/285672507_Structural_characterization_and_functional_evaluation_of_an_exopolysaccharide_produced_by_Weissella_confusa_AJ53_an_isolate_from_fermented_Uttapam_batter_supplemented_with_Piper_betle_L_leaves
Regards,
Ashwini Dubey
Article Structural characterization and functional evaluation of an ...
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