As C/N is very critical in the optimization of the Exopolysaccharide production by Microorganisms. My concern is that I have used complex media such as tryptic soy broth (TSB) for other optimization experiments. TSB medium contains glucose as carbon and tryptone as the nitrogen source. Should I use different ratio of glucose and tryptone to optimize C/N ratio? Any suitable reference paper please.
Thanking in anticipation
Dear Ali
You can read this paper: Article Cultural Optimization of a N ew Exopolysaccharide Producer “...
Dear Ali, You can use modern statistical tools like Response surface methodology (RSM) for optimization purpose.
Few references are:
https://doi.org/10.1016/j.ijbiomac.2016.09.007
https://doi.org/10.1016/j.carbpol.2012.06.021
You can find related articles from references quoted in these researches.
Dear Ali. To solve your problem, a multivariate analysis is necessary. And as recommended by Sarmad A. Qamar Response surface methodology (RSM). But in view of the complex substrate, it will be difficult to provide a given level of nitrogen.
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