Looking for a mindful parenting scale?

Hello, I am looking for a mindful parenting scale to use with a cohort of parents (predominately mothers) who have personality disorder related difficulties, with a preference for measures with...

06 September 2020 6,573 5 View

How do you deal with multiple submissions to a survey from the same respondent?

Hello, I am a graduate student and I am working on cleaning survey data. When cleaning the data I realized that a few respondents completed the survey twice. I reviewed the survey data to see if...

04 July 2020 5,688 8 View

How can I create an environment in MATLAB or Python to test a traction control algorithm?

For a project I am working on I need to implement traction control to a RC car. In designing this algorithm I need to create a simulation environment in order to test the control algorithm. The...

24 April 2020 8,529 1 View

Why my proteins do not elute from DEAE-52 column?

I have tried pH from 5-8 and NaCl concentration from 0-2 M. But my protein mixture bind firmly on the column.

14 March 2020 7,170 2 View

Does Casewise diagnostics in SPSS provide the variables with a standard deviation of your selection based on the mean? Or anything else than the mean?

Good day Does SPSS Casewise diagnostics produce the variables which are a chosen standard deviation from the mean? Or are these standard deviations calculated based on another item? I read an...

13 December 2018 9,899 5 View

Can ammonium sulphate acidify sterile media?

I recently did an experiment where I created sterile media by adding 1mM of ammonium sulphate to Murashige and Skoog (MS) Modified Basal Salt Mixture (No Nitrogen) M531. The media was not buffered...

30 October 2017 3,574 4 View

Protein extraction from micro-dissected tissues?

10 April 2016 8,693 2 View

Why does most commercial RNase A lyophilized powder have DNase activity?

14 March 2016 1,273 1 View

For Micro-dissected tissue fixation, is it better to use Formalin or Paraformaldehyde and for how long should I incubate for ?

07 March 2016 4,117 5 View

How do I control time incrementation in Abaqus using User Subroutines?

I am currently incorporating a fatigue user defined material model (UMAT) within Abaqus to describe the constitutive behavior of a cohesive zone element. I know the required psuedo time (elapsed...

01 November 2015 9,666 10 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View