What test should I use?

15 February 2021 911 5 View

How remove endotoxins from DEAE-Sepharose?

Hi to everyone! I have to validate endotoxins removal from DEAE-Sepharose I use for the purification of my hormone. The resin undergoes to a sanitization protocol with NaOH. To demonstrate the...

29 October 2020 9,141 4 View

How can I roughly estimate the current denisty in an FPGA?

Hello, I am currently working on the estimation of the electromigration of an FPGA. We just performed the construction analysis so we know all the values of wire width and height for each metal...

22 June 2020 2,743 3 View

What is the (average) DNA yield (ng) from one single nematode?

Do you know where I can found published information about DNA quantity (in ng) from one single nematode? Any publications/books to suggest me? I Know it seems a simple and basic information, but...

31 March 2020 6,710 1 View

Can I use Arrhenius Relationship to determine Accelerated factor , when test temperature is lower than use temperature?

Hello, I have a question regarding the acceleration factor. We know that the lifetime (TF) of a component can be expressed as: (1) TF=A0exp(Ea/(kT)) where A0 is a constant (component...

01 March 2020 6,635 6 View

Does anyone have experience with fMLP as a neutrophil chemoattractant in a transmigration assay?

I am planning to perform a transmigration assay (transwell plate) in which are involved neutrophils , therefore I will use fMLP as a chemoattractant. I did find some papers that describe "The...

19 February 2020 5,855 3 View

Where to publish a short descriptive note about a novel behaviour?

I have found a novel behaviour in an insect and I would like to publish it, but I do not know where to publish something that is just descriptive (with photos and videos) and does not includes any...

13 June 2019 401 1 View

Is there a pigment that can be used to dye PMMA in anisole?

I am looking for an organic dye, soluble in anisole to be able to get coloured PMMA films

30 April 2019 812 1 View

Simultaneous fixation, permeabilization and Phalloidin staining of adherent cells?

Dear all, does anybody have experience with the simultaneous fixation, permeabilization and Phalloidin staining of adherent cells (fibroblast or endothelial cells/cell lines)? Many companies...

25 June 2018 2,381 4 View

Anova significative and no significance revealed by Tukey HSD?

Hi all, I am doing ANOVA to compare tree different groups, ( n= 22,25,23). each group have normal distribution and ANOVA result significative (p= 0.03). ANOVA residuals are no- significatives...

22 April 2018 4,013 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View