Valeria Balmaceda

5 Questions 3 Answers 0 Followers

Questions related from Valeria Balmaceda

What type of proteasome inhibitors is better to use in tissue lysate?

Hi all. I am looking for a proteasoma inhibitor to use in my tissue lysate. I found MG132; lactacystin and PR39. Anyone know which of these is more effective to use in a tissue lysate?, Do I need...

17 April 2020 4,295 3 View

Anova significative and no significance revealed by Tukey HSD?

Hi all, I am doing ANOVA to compare tree different groups, ( n= 22,25,23). each group have normal distribution and ANOVA result significative (p= 0.03). ANOVA residuals are no- significatives...

23 April 2018 4,198 2 View

Does anyone have a protocol to measure GPD?

Hi all, I am looking for a protocol to glycerophosphate dehydrogenase to assay enzymatic activity on cryosection of soleus muscle. I tryed whit one using NBT and PMS but I did not have any...

27 March 2018 2,160 3 View

Could water interfere in DAB reaction on IHC?

Hello alI. I ´m doing IHC on brain slices fixed on paraffin, and I have a question regarding with DAB reaction. so.. When I prepare DAB reagent on water ( DAB 1%+ 0.03% H2O2 all in mq water)...

23 February 2018 3,463 1 View

Chip PCR Problems

After IP and reverse cross linking and DNA purification, I have done a standard semicuantitative PCR using control primers (GAPDH), which I expect bands around 166 bp. In the results of my...

06 June 2013 9,199 0 View