Usually fuzzy fuzzy things (mold fungi?) might grow if sugar solution is stored for a long time. If such sugar (e.g., glucose) solution is applied to examine bacteria and fungi in environmental samples (e.g., soil), how would the molds in solution affect the native bacteria and fungi? Thank you.
I would rather suggest that you prepare glucose solution freshly with every experiment which has been filter sterilized through a 0.22 um filter. By adding the contaminated glucose solution you would create an environment where the contaminant would be at higher levels than the bacteria and fungi present in your sample. This would create an environment where the contaminant will out compete with the bacteria and fungi within your sample either by using up all the nutrient or the production of antimicrobial compounds. Regarding the DNA extraction, using such an contaminated solution would create a lot of background DNA which would result in certain species DNA being out masked if present at low concentrations.
I would rather suggest that you prepare glucose solution freshly with every experiment which has been filter sterilized through a 0.22 um filter. By adding the contaminated glucose solution you would create an environment where the contaminant would be at higher levels than the bacteria and fungi present in your sample. This would create an environment where the contaminant will out compete with the bacteria and fungi within your sample either by using up all the nutrient or the production of antimicrobial compounds. Regarding the DNA extraction, using such an contaminated solution would create a lot of background DNA which would result in certain species DNA being out masked if present at low concentrations.
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