Dear Preethi Sridharan

Pls. find the attached files, may help in your research good luck.

https://www.researchgate.net/post/Why_do_my_monocyte-derived_THP-1_macrophages_die_so_much

https://www.researchgate.net/profile/Kyoung_Kim2/publication/6471426_Optimized_THP-1_differentiation_is_required_for_the_detection_of_responses_to_weak_stimuli/links/55de4e0c08ae45e825d39b32.pdf

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545815/

http://www.bio-protocol.org/e1638

http://www.jove.com/video/51960/highly-efficient-transfection-human-thp-1-macrophages

 https://www.bdbiosciences.com/documents/BD_Multicolor_MonocyteMacrophageDiff_AppNote.pdf

http://download.springer.com/static/pdf/339/chp%253A10.1007%252F978-3-319-16104-4_14.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Fchapter%2F10.1007%2F978-3-319-16104-4_14&token2=exp=1466234366~acl=%2Fstatic%2Fpdf%2F339%2Fchp%25253A10.1007%25252F978-3-319-16104-4_14.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Fchapter%252F10.1007%252F978-3-319-16104-4_14*~hmac=95c93b382fda40973602b15a3c3035e032884333e75082f22f43996427cfa0b4

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0008668

http://www.hindawi.com/journals/mi/2013/697972/

http://www.bio-protocol.org/e1638

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC96928/

Regards

Prof. Houda Kawas

Article Optimized THP-1 differentiation is required for the detectio...

Thanks prof. houda I have gone through some of the links you have provided but nowhere it is mentioned that which type of plate should be used either adherent or suspension they have just mentioned that they have used 24-well plate/96-well plate 

Dear  Preethi Sridharan

Thank for your,  all  those not mentioned the type just because it could be no differences between them .

Regards

Houda

Thanks Prof. Houda 

I think it would depend on what you are going to do with them once they've differentiated. I would suggest you do the differentiation in whatever size well you will use for the assay you'll do with the differentiated cells. If you are going to transfect them, then use a 100 mm petri dish so you have lots of cells to transfect.

Hello David,

Thank You very much for your response. My doubt is regarding the material of the plate whether it should be adherent/suspension because the monocytes are suspension cells and after differentiation to macrophages they will adhere so I don't know if I seed the monocytes in suspension dishes/well plates after differentiation the macrophages will adhere to them. 

Yes, the macrophages will adhere to the bottom of wells after differentiation. 

I have tried two different brands of the plates in my experience so far. I am using Corning® Costar® 24-wells and 96-wells, flat-bottom and tissue-culture treated now. 

Interestingly, if you check https://www.lgcstandards-atcc.org/Products/All/TIB-202.aspx#culturemethod, they specifically recommend Corning® tissue culture flask for culturing.