What is the highest concentration of inhibitors that can be tested on purified LOX enzyme in a quartz cuvette to study enzyme UV-kinetics inhibition?

I am studying the enzyme kinetics of 5-LOX using UV to detect formation of conjugated diene products. I am curious to know if it's okay to test compounds at 50-100 μM in a UV-kinetics study?

24 October 2013 7,018 8 View