Preethi Sridharan

5 Questions 16 Answers 0 Followers

Questions related from Preethi Sridharan

What type of dishes/ well plates should be used for THP-1 differentiation to Macrophages?

I am trying to differentiate the THP-1 cells to macrophages using Vitamin D3 and would like to know during treatment which type of dishes/ well plates should be used whether adherent / suspension?

18 June 2016 6,893 7 View

Is there any chemical compound available that activates HIF-2 alpha protein expression? or that stabilizes its expression?

Like cobalt chloride stabilizing HIF-1 alpha is there any chemical compound available to induce/ stabilize HIF-2 alpha? or is there any specific physiological hypoxic condition ( % O2 or % N2 or %...

05 December 2015 8,581 8 View

Could someone help me with the protocol for differentiating THP-1 cells using vitamin D3?

I am trying to differentiate THP-1 cells using vit d3 could someone give me the protocol for the complete differentiation. Is there any staining method available to ensure the differentiated cells...

19 September 2015 8,015 4 View

Which is best reference gene for Real-Time PCR gene expression studies under chemical hypoxic condition?

I am trying to study the expression of my gene of interest under hypoxia (chemically induced) there were many reports that states that GAPDH and Microglobulin Beta are more stable genes that can...

04 August 2015 1,887 4 View

Why do I observe 4 bands of total RNA?

I do total RNA extraction from U87-MG cells and convert them to cDNA which I eventually use for gene expression studies but when I check the integrity of RNA by agarose gel it is showing 4 bands....

23 July 2015 7,879 9 View