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I am trying to differentiate the THP-1 cells to macrophages using Vitamin D3 and would like to know during treatment which type of dishes/ well plates should be used whether adherent / suspension?
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I am trying to differentiate THP-1 cells using vit d3 could someone give me the protocol for the complete differentiation. Is there any staining method available to ensure the differentiated cells...
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I do total RNA extraction from U87-MG cells and convert them to cDNA which I eventually use for gene expression studies but when I check the integrity of RNA by agarose gel it is showing 4 bands....
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