DNA isolation hindered for long term frozen leaf samples;Why?
Is we have any raw sample extract that show positive confirmation in ninhydrin test?
01 February 2019 7,919 5 View
What are the application of thermodynamics (Physical Chemistry) in Biotechnology ?
07 August 2018 3,328 9 View
Job opportunities after perceiving a Doctorate degree in biotechnology in India presently?
02 March 2018 931 3 View
What were the general inferences concluded after the phylogeny reconstruction?
10 November 2017 7,978 2 View
Bootstrap value X mega software
09 October 2017 8,550 2 View
Say for example a combination of MATK and RBCL gene in plants ?
09 October 2017 1,566 3 View
How can we get a single sequence from two separate forward and reverse sequenced PCR product ? Is it possible by bioedit software?
08 September 2017 5,862 2 View
Can anyone explain it with an example and steps followed?
08 September 2017 1,735 4 View
How does pollen-pistil incompatibility and geographic barriers between plant species in a family favors / disfavor horizontal gene transfer ?
06 July 2017 9,232 3 View
Why transition from moneocious to Dioecious and different forms of Dioecious were found in some plant families? Is this an evolutionary adaptation or due to some other reasons?
06 July 2017 2,006 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
