I am using 10% FBS RPMI medium with 1% Sodium Pyruvate, penicillin streptomycin and MEM non-essential amino acids. If I use 20% FBS the cells become giant vacules rather then proliferating. I split them every 6-7 days. They are nearly impossible to work on or do an experiment with this proliferation rate. What can I do?
Hi- I strongly suggest to follow ATCC guidelines for handling HCC1937 cells. I had similar problems with ongoing MCF-7 line. Following ATCC's instructions greatly helped. See link.
https://physics.cancer.gov/docs/bioresource/breast/NCI-PBCF-CRL2336_HCC1937_v1.0_SOP-508.pdf
Also, the same issue with HCC1937 was previously discussed in Researchgate. See link below to follow the thread. This might help too.
https://www.researchgate.net/post/Did_anyone_experience_problems_with_extremely_slow_growth_and_poor_attachment_of_HCC1937_breast_cancer_cell_line
Best wishes.
1. Split ratio in ATCC could help.
2. Do a mycoplasma test-vacuoles are good indicators of that in addition to other stress.
3. Check the recommended supplements such as vitamins, L-glutamine etc.
I had similar problem with SHSY5Y neuroblastoma cell-line. Later I figured out two possible reasons:
1.pH of media used
2.quality of FBS used in the culture medium. You can try to change these and see if the cell grows.
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