What to do if the gene we are interested does not give positive staining?

25 September 2020 1 9K Report

Hi, I am designing my proposal on gene which cause chemoresistance in cancer. From literature review I have come up with a gene COL11A1 which has previously been identified as a chemoresistance biomarker. I want to know that what if I do not receive any positive result of staining then what measure will I do to atleast get guarantee that something to work on?

Lianzheng Zhao

If you have tried both IF or IHC staining without positive signals, your antibody may be not good enough or there is no expression of your interested gene. You can take some positive samples as control to make sure your protocols.

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