What thickness do you cut mouse brain slices on a microtome and what blade is preferred?

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Is anyone researching the relationship between learner engagement and employee engagement?

Is anyone researching the relationship between learner engagement and employee engagement? I would be curious to read what you have found on this topic if I haven't already read it. Thanks in advance.

02 March 2024 2,030 0 View

How do you stain frozen muscle sections with p16 or p21?

I need to stain frozen sections with 2 different stains (p16 and p21) which has been done in the past on FFPE blocks. Any advice or updated protocol will be much appreciated.

14 September 2022 9,179 0 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 3,417 1 View

Permission to use the Nurse's Spiritual Care Practice and Perspective?

Dear Martha, I am interested in doing a class to educate nurses how to assess spiritual distress and provide basic spiritual care to patients in the hospital. As part of the class wanted to have...

30 January 2020 9,044 16 View

Is anyone working on market information systems and evaluation of impact on production, yeild and income ?

I am working on horticulture crops, with defined number of farmers, who market at a known formal market. Does anyone have reference to recent work in this direction? What were the lesson and...

17 November 2019 9,434 1 View

Is there information regarding using GLP-1 medications post bariatric surgery?

WOrking in bariatric clinic, with diabetes managment.

11 July 2019 9,668 5 View

Turbo GFP lentivirus is working on HEK cell but i could't find turbo GFP signal in vivo OE tissue(nasal infection). why?

Hi guys as i described in the question, i generated lentivius and i used pGIPz vector from Dharmacon. i checked it's working efficiency on the HEK cell line but after i nasal infect the virus, i...

28 March 2018 8,024 1 View

Hi am researching for my dissertation and am looking for research on the HOT algorithm. Can you reccomend any?

Dissertation is questioning whether CPR is the main priority in a traumatic cardiac arrest.

24 January 2018 2,601 3 View

What statistical tests do you suggest?

I am going to educate staff on health literacy awareness. I will do pre- and post-test. For this, I plan on descriptive statistics and t-test. Is this correct? After beginning advance care...

23 September 2017 7,261 3 View

After the first year, what is the status of your goal to develop talent pools for the executive nurse?

My curiosity stems from my personal journey as a nurse in transition.

02 July 2017 9,656 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Can telekinesis, telepathy, and prediction be achieved through science?

Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...

28 July 2024 7,522 0 View