Thoria Donia

9 Questions 21 Answers 0 Followers

Questions related from Thoria Donia

What the best way to sterilize a nano-formulation before adding on cells?

Please, how can I sterilize a nano preparation if it is affected by light, and it is in colloidal state and may lock the syringe filter? Later I have to study its effect on cultured cells....

19 March 2023 1,179 1 View

Multiplex PCR primer with mixed nucleotide residues?

Dear all During reading in some papers about multiplex PCR primers, I have found some letters within the sequence as d, m, w and h which are referred to some bases sometimes one of three bases....

20 May 2022 4,770 2 View

What is the suitable solvent for synthetic organic compound to be checked for biological activity?

Hello everyone I have dissolved organic compound in 100% DMSO in high concentration to check its biological activity on cancer cells after diluting it in media. Once it was added to media for...

28 July 2018 8,171 5 View

No signal at all in DNA sequencing ?

I always do sequencing reaction for plasmid after subcloning of a specific sequence ( and to confirm subcloning I did restriction digestion and gel electrophoresis and get bands) but no signal...

06 March 2017 7,521 5 View

Do you have an explanation for a decrease in AST and ALT activities in fibrosis model induced by CCl4?

Really I have a decrease in AST and ALT activities in liver fibrosis induced by CCl4 in a study in which 3ml /kg CCl4 40% in olive oil twice a week for 5 weeks and I can not find a cause but I...

06 September 2015 2,411 8 View

What are the steps for analyzing gene polymorphism of a specific gene in rat?

If you are going to study gene polymorphism of specific gene in serum and liver of rats after rat experiment and this gene may be affected but you do not have primer sequence to amplify that gene...

31 July 2015 2,847 3 View

Could you please help me to know how to crystallize hesperidin?

in extraction of hesperidin from orange peels methanol was used and then acetic acid on concentrated methanol extract but on trying to dissolve the powder after evaporation of acetic acid in...

31 July 2015 8,031 3 View

Could you please suggest a method to identify both bacterial and fungal proteases?

Is there a simple and specific method for determination of fungal and bacterial proteases acting on peptide bond at tyrosine and asparitic acid residues respectively when trying to estimate its...

14 July 2015 7,815 6 View

What the normal range of serum adiponectin in rats?

What is the normal range of adiponectin in rat sera in ng/ml and to what value it will increase in different diseases cases as its estimation has specific linearity and if the method is specific...

30 June 2015 2,238 3 View