Thoria Donia

4 Questions 13 Answers 0 Followers

Questions related from Thoria Donia

What the best way to sterilize a nano-formulation before adding on cells?

Please, how can I sterilize a nano preparation if it is affected by light, and it is in colloidal state and may lock the syringe filter? Later I have to study its effect on cultured cells....

19 March 2023 1,045 1 View

Multiplex PCR primer with mixed nucleotide residues?

Dear all During reading in some papers about multiplex PCR primers, I have found some letters within the sequence as d, m, w and h which are referred to some bases sometimes one of three bases....

20 May 2022 4,677 2 View

What is the suitable solvent for synthetic organic compound to be checked for biological activity?

Hello everyone I have dissolved organic compound in 100% DMSO in high concentration to check its biological activity on cancer cells after diluting it in media. Once it was added to media for...

28 July 2018 8,072 5 View

No signal at all in DNA sequencing ?

I always do sequencing reaction for plasmid after subcloning of a specific sequence ( and to confirm subcloning I did restriction digestion and gel electrophoresis and get bands) but no signal...

06 March 2017 7,440 5 View