Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

13 January 2021 8,372 3 View

What is the sampling technique used in an online survey?

I am asking about the sampling technique used in an online survey. The survey will be distributed online via social media channels. We are targeting adolescents. The sample size will be estimated...

11 July 2020 8,685 14 View

Can I leave blot in block buffer for 48 hours?

Is it ok ti leave the blot in non fat milk for 48 hours in +4 degrees or is it better to leave it tbs-t for that extra 24hrs?

23 February 2020 7,475 2 View

Should the HCV Core protein fused with His-tag (without linker) be extracted in the insoluble or soluble protein fraction?

I have infected insect cells SF9 with recomb. baculovirus expressing Core.His and another with GFP. I have extracted the soluble proteins after 3 days and did western blot using anti-his,...

10 February 2020 2,359 4 View

How to quantitatively analyze a western blot for a target protein with multiple specific bands?

Usually in western blot there is a single band for a target protein and we can measure its density and calculate with reference to an internal control for normalization. the problem occurs when...

03 April 2018 1,738 6 View

Seismic Dynamic Ground response analysis using ABAQUS, results not matching with deepsoil?

I am trying to replicate the results from deepsoil for an earthquake signal for a simple soil column model on ABAQUS. I have used finite elements in the direction of loading. However, in ABAQUS...

02 March 2017 1,029 0 View

Dynamic Soil-Pile Interaction; Why is the soil deforming 0.5 m vertically during shaking?

I modelled a helical pile (with a mass at the head) installed in dry sand undergoing seismic shaking at the base. I am trying to mimic a full-scale test we performed in a shaking table. I am using...

02 March 2017 3,926 8 View

Dynamic Soil-Pile Interaction using ABAQUS, question regarding modeling pile?

Hi everyone, I am trying to model a steel helical pile with a mass on top in a soil block that I will perform dynamic analysis on (by applying a time history on the base). For now I am trying to...

31 December 2016 6,681 10 View

Analyzing a gaussian output

Hi everyone I did an opt+fre calculation using gaussian 09 , now I have the output file but I don't know how to analyze and interpret my results (HOMO-LUMO and NBOs ) can anyone help?

01 January 1970 8,907 4 View

How can I view my work " paper" and save my idea, while the publishing process takes a long time

If I have a new work and isn't submitted yet to any journal or conference. can I put it in arXiv and what is the advantage and disadvantage of this step?

01 January 1970 3,209 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View