I am using Tetrazolium (INT) as a viability stain for AMF spores but I am finding some inconsistencies in the results I obtain. Any other technique that I could use to contrast my results with INT? Thanks for your answers.
In my opinion, the best way is to use the plant test technique in a sterile soil, i.e inoculate the test plant (e.g, onion) with the mycorrhizal fungal spores then determine the mycorrhizal infection percent in roots, you will find a linear relationship between the viability of the mycorrhizal fungal spores and the root infection percent.
Good luck
I was thinking in a less time-consuming technique than a bioassay. Thanks anyway.
You can use vital stains, for instance:
http://en.wikipedia.org/wiki/Trypan_blue
But I don't think you can completely eliminate inconsistencies... it's not a strong side of such techniques
In my opinion , staining techniques to assess the viability are not accurate. One way to assess its viability , is the capacity to colonize the roots , so I agree with the view Mr.Abu-Bakr
I am already conducting bioassays to study the inoculum viability. However, I was thinking in some other way to analyze it, less time-consuming. I think I will try to develop an alternative technique... ;) Thanks a lot for all your answers.
We are using MTT staining of mycorrhiza spores and IP by root staining
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