I have checked my solution with UV/VIS by adding 5X Bradford reagent. But it shows a red shift which means results are not appropriate. Because the range of bradford 5X is upto 1000 ug/ml. And results should be between 400 to 600 nm.
What other method i should have to use for high concentration protein amount detection???
you can dilute your sample solution to bring in the workable range, or you can use a lesser sensitive technique , e,g NMR , for quantification.
Debopam Ghosh... in my research i am making a protien sensor and i have to detect that how much protien is detected with respect of time by sensor.
If i dilute my sample, it will not effect my sensing values???
true, in that case i suggest you use an alternate of Bradford 5X, one that has a higher linear response range, please look into relevant literature on synthesis and designing of these spectroscopic reagents for protein quantification.
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