What surface proteins can be targeted to distinguish pDCs in Syrian hamster and Peromyscus mixed-cell population cultures (BMC-derived)?

09 December 2015 1 3K Report

Isolated BMC stimulated with recombinant mouse FLT3-L in 10% FBS RPMI. What techniques can be used or surface proteins targeted to distinguish presence of plasmacytoid dendritic cells?

Jean-Jacques Pin

Three main surface markers are studying: CD 123, CD 303 and CD 304.

By varying the isoforms of each of these markers, we can divide the different populations of DCs from blood, and subtypes of pDCs.

The CD123 has at least two isoforms, can distinguish the two populations of pDCs.

The CD304 also has two isoforms that identify two populations of pDCs.

The CD303 has over six isoforms that allow increasingly differentiate healthy pDCs of pDCs from pathological tissues.

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