Is secondary fixation necessary for processing plant materials for SEM?

I am trying to create a protocol for several fixation methods for Scanning electron microscopy. using FAA, glutaraddehyde and ethanol as fixatives.

11 November 2019 7,215 1 View

What non-parametric statistical analysis can you use for small sample sizes, n=2, and still show statistical significance?

I am doing a project on a therapeutic group, utilizing non-parametric ordinal data to account for changes in emotional distress. Unfortunately attendance has been somewhat inconsistent throughout...

23 March 2019 9,948 14 View

How does the UVF parameters affect the quality of heavy oils?

I have data regarding UVF parameters, which include Fluorescence Intensity Values (i) in Relative Fluorescence Units (rfu). Attached in the excel file is the UVF parameter list and its standard...

16 May 2018 2,636 1 View

What is Mesenteric Cancer?

What is Mesenteric Cancer? What is the root cause of Mesenteric Cancer? How can we cure or prevent Mesenteric Cancer?

20 October 2017 5,744 4 View

How would I analyze and present longitudinal patient data, which is split into 2 or more samples?

12 July 2017 8,424 2 View

Effect of flash freezing of pelleted cells?

20 February 2017 7,949 2 View

How to directly test viral primary antibody?

I have a polyclonal anti-MODV antibody as well as a monoclonal anti-flavivirus (E protein) antibody and would like to test both against MODV in cell culture. In developing a protocol I would like...

14 January 2017 5,944 4 View

Can we use Big Data Analytics to Automate / Replace Manual Literature Review that are conducting by Researchers?

14 October 2016 6,049 7 View

Can we use Big Data Analytics to Analyze & Derive New Areas for PhD Research?

14 October 2016 6,636 13 View

Have anyone encountered PMMA cracking or charging issue on hBN flakes after development?

I create patterns (several micrometers) on hBN flakes on a Si/SiO2 substrate using EBL (100kV). I find cracking / extra lines emerging from my pattern after development. I guess it is due to...

02 July 2016 2,355 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View