Looking for any freeware on potential drug candidates screening. Any advice please.
Hi all, I have a ex vivo drug screening system for leukaemia, but I realised some samples contains both leukaemia and a significant amount of healthy cells (5-30%). I am looking for a no-wash (or...
04 June 2024 3,779 0 View
Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...
30 May 2024 7,604 3 View
I'm interested in models of CPD that take into account both of these key criteria.
19 March 2024 4,482 1 View
Hi all, I am currently helping my colleague to do a drug screening assay. We have use dTag to "turn off" the protein expression of our protein of interest and treat the cell line with drugs...
11 January 2024 8,422 2 View
Hi all, I have been doing drug screening on clinical samples and multiple 384-well plates are used for each samples. However, when we look at the cell number in the DMSO control on each plate,...
05 December 2023 4,478 7 View
Specifically, I am wondering if I can predict the surface marker expression of CD14 and CD16 on monocytes based on scRNAseq data that includes the expression level of those two genes. Does anyone...
29 March 2023 6,818 3 View
Hi Community, I am planning to express 6 human proteins in a mouse cell line. They are from 400 to 800 bp long. I want to use three vectors, each containing two CDS connecting with 2A peptides,...
04 February 2023 3,165 1 View
Hi Fellow Researcher, I understand the title isn't clear enough. Allow me to explain. I have two pairs of cell lines (pair 1: cell line A, cell line A with WT gene 1; pair 2: cell line A, cell...
11 January 2023 7,213 1 View
Please advise any good startup companies which mainly based on benzimidazole derivatives drug development, better at late stage of pre-clinical or Pre-IND stage. Thanks
28 December 2022 1,808 0 View
There seems to be much previous interest in developing haemodynamic monitoring equipement, espcially non-invasive ones, for haemodialysis. However, there is not much over the last decade. Can...
21 December 2022 1,359 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
EDX analisys
31 July 2024 5,947 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View