Tyler Sherman

5 Questions 6 Answers 0 Followers

Questions related from Tyler Sherman

Possible to conduct PCR using hematocrit sample?

Does anyone have experience running an RT-PCR for pathogen detection from samples of (deer) hematocrit? A collaborator is offering the spun blood tubes left over after sera collection (used for a...

22 July 2022 1,961 0 View

Is it absolutely necessary to use "fecal specific" DNA extraction kits for sampling the microbiome?

I would like to extract total bacterial DNA from stool samples for 16S amplicon sequencing... I have a bacterial DNA extraction kit that is intended for use with lab-grown bacterial cultures, but...

18 May 2019 970 3 View

Has anyone conducted a successful plaque assay with Modoc virus?

Still searching for a protocol that will work. Thanks!

01 August 2017 8,558 2 View

How to directly test viral primary antibody?

I have a polyclonal anti-MODV antibody as well as a monoclonal anti-flavivirus (E protein) antibody and would like to test both against MODV in cell culture. In developing a protocol I would like...

15 January 2017 6,091 4 View

What surface proteins can be targeted to distinguish pDCs in Syrian hamster and Peromyscus mixed-cell population cultures (BMC-derived)?

Isolated BMC stimulated with recombinant mouse FLT3-L in 10% FBS RPMI. What techniques can be used or surface proteins targeted to distinguish presence of plasmacytoid dendritic cells?

09 December 2015 3,206 1 View