Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How can I indicate to you that I am in fact the author of a book that you have informed me about for quoting other work of mine?

The question concerns this title: Viktor Smith Naming and Framing: Understanding the Power of Words across Disciplines, Domains, and Modalities. Routledge. All the best, Viktor

01 January 2021 4,197 1 View

50% nitric acid bath container?

We're using a fluorometer (Horiba Aqualog) to analyze fluorescent dissolved organic matter (FDOM) in water samples. The samples are analyzed in quartz cuvettes, which must be bathed in a solution...

17 November 2020 7,236 2 View

Where can I find the RG Impact Score for a journal?

I am debating where to submit a paper and would like to compare journals' impacts.

09 November 2020 6,962 4 View

Grounded theory or Interpretive phenomenological analysis? Which is best for dissertation studying perinatal mental health?

I am curious what the differences are between using grounded theory or interpretive phenomenological analysis (IPA) for my dissertation. I am seeking to both complete my dissertation for...

29 October 2020 3,066 4 View

Multilevel model for change- using time-varying variables at different waves to outcome?

I am using the multilevel model for change. I am using UKHLS youth survey where an outcome for wellbeing occurs at waves 2,4,6 and 8. I have predictors such as computer screen usage and many...

28 September 2020 3,984 1 View

Are human circadian rhythms influenced by electromagnetic radiation? Why does the literature suggest no significance when relating to melatonin?

I understand that light is one of the strongest external cues to affect our clock system. I am curious about other possible factors that are less discussed. I am finding a great amount of...

03 August 2020 8,586 2 View

What are the independent, intensive parameters for multicomponent mixtures (and how do I determine them)?

Hi all, For different problems in gasdynamics/thermofluids, different thermodynamic inputs are most convenient for expressing and solving the problem. For example, if I have a stagnation process,...

23 June 2020 1,085 2 View

Is it okay to use latent variables in the SEM model that have different constraints than CFA for individual latent variables?

I am conducting a structural equation model with three latent variables containing three indicators each. The latent variable CFA's are significant with constraints on 2/3 indicators, but the SEM...

28 April 2020 3,275 4 View

How to culture of keratinocytes from human skin punch biopsy?

Hi, I have been having problems culturing keratinocytes from 4mm human skin punch biopsies. When I recieve the biopsy I first was the samples in sterile PBS, then I remove the subcutaneous fat. I...

24 April 2020 2,696 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Three-way robust ANOVA in R?

I am trying to conduct a three-way robust ANOVA in R using the WRS2 package as: t3way(Stem ~ Species*Temperature*Salinity, data = tutto) and there is an error saying : Error in if...

22 February 2021 1,674 8 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

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22 February 2021 9,932 11 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View