I am working on the project "Post harvest losses of Maize and wheat by Fungi" .
Dear college:
You know that each fungus has its specific carbon and nitrogen requirements for sporulation. However, potato dextrose agar (PDA) is the most widely used medium in fungal isolation and cultivation. Other commonly used media include potato carrot agar, malt dextrose agar, yeast phosphate extract medium, Czapek yeast autolysing agar, malt extract agar, cornmeal agar, potato sucrose agar, etc. These media provide favorable growth conditions for most endophytic and pathogenic fungi, but are often ineffective in inducing sporulation of sterile isolates.
I suggest you try all this kind of medium first. If it is not successful, try to reduce the concentration of nutrients in the media, in my case, I tried to observe the conidia of Curvularia kusanoi and I did not see them in any of these media, I only saw them when the microorganism was grown in water agar.
i hope these can help you!
One of best sporulation techniques used by Fusarium researchers is the use of carnation leaves. I do agree with Maryen Alberto that rich media can be problematic as there is often too much mycelium and it does help the sporulation and its visualization.
Fisher, N,, et al. 1982. Carnation leaves as substrate and sporulating cultures of Fusarium. Phytopathology 72(1):151-153.
This technique is used by Fusarium researchers for sporulation needed for identification and characterization based on spore morphology. The gas sterilized leaflets are placed on sterilized water agar and the cultures inoculated with pure culture isolates.
carnation leaves is the best substrate to get perfect and standard spore for Fusarium specially if you are doing taxonomy study.
we used 2 media: carnation leaf agar (CLA) and SNA @Temesgen Assefa Gelaw
I use water agar medium CLA (carnation leaf agar) which promoted good growth, sporulation, and maintenance of the original cultural type of the Fusarium spp. The reason I use CLA is to avoid the problems of colonies losing pathogenicity and delayed sporulation with rich media such as Potato Dextrose Agar, (PDA)@Temesgen Assefa Gelaw
CDA Medium may also try. I got best sporulation of Fusarium solani. For sporulation less nutrition requires and one third plates be poured with medium .
I have no experience to work on Carnation Leaf Agar. We isolated Fusarium solani from clinical a s well as environmental samples on Sabourdaud dextrose agar with chloramphenicol, Pal sunflower seed medium, and APRM agar.The later two media were developed by us.
SNA media is very clear especially on the observation of the resting structure Chlamydospores
Thank you everybody for your constructive Response and all the idea was found to be nice.
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