Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

How could I retrieve view collection on Epi Info 7 when Microsoft jet database engine could not find the object 'Databases'?

What should I do for the following error message to open my project file on epi info 7? Error: Could not retrieve view collection. The Microsoft jet database engine could not find the object...

30 July 2020 9,175 1 View

How to perform sequential analysis of earthquake then fire in abaqus?

Am doing a study on the Post-Earthquake Fire Performance of Reinforced Concrete column with surface reinforcement using abaqus. I need to know the procedure to do the sequential analysis in...

28 July 2020 9,759 2 View

Current research topics on hydro power planning and design ?

research topics on hydro power development related to design, planning or hydrology aspect

03 January 2020 406 4 View

Is it matrix strength or composite material strength?

Composite material made from a layer of fabrics (two woven fabrics connected with matrix), when the composite layers separated each other rather than break under tensile strength test. Is the...

23 December 2019 231 9 View

Is the reaction condition different in the powder and pellet form of same composition ceramics heat treated at the same condition?

XRD of calcined MPB composition PZT powder showed pure perovskite phase but that of sintered pellet of the same composition at the same temperature showed some extra phase, possibly the extra pick...

27 January 2019 1,886 5 View

Which Structural Equation Modeling software is relatively more comprehensive and therefore recommended for most research situations?

Which Structural Equation Modeling software (eg., LISREL, AMOS, EQS... etc) is relatively more comprehensive and therefore recommended for most research situations? And why? Please help me on how...

04 December 2015 5,419 8 View

Is there a standardized measuring scale for children psychological well being?

16 October 2015 4,916 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View