to study malaria resistance gene which type of primer can i use?
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Salt water does not evaporate faster than fresh water; in fact, fresh water always evaporates faster than salt water. This is because of the difference between the salt and water molecules. Water...
31 March 2024 6,271 1 View
During a separate inquiry, I encountered the examination of sulfate ion concentrations. Although Spectrophotometric sulfate measurement is suitable for low concentrations between 1 mg/L and 40...
08 February 2024 5,663 2 View
My article entitled "Exploration and collection of root and tuber crops in East Wollega and Ilu Ababora zones: Rescuing declining genetic resources" is highjacked by some profile in Research gate...
28 December 2023 7,801 3 View
When extruding in " Design Modeler", we have two options. Add material or add frozen... Any ideas, welcome!
19 December 2023 7,946 4 View
Precision agriculture is the use of data, sensors, and technology to optimize crop production, reduce environmental impact, and increase profitability. It is one of the most promising and...
02 August 2023 7,539 3 View
What are the potential mechanisms behind the beneficial effects of plasma treatment on wound healing and tissue regeneration? How can we optimize plasma parameters to enhance these effects?
18 May 2023 5,060 3 View
Hello! I was doing research on the solar collector (evacuated tube solar collector with bottom reflector). And something bothered me. Most of the studies are solar panel tilt angles. Can I take...
10 May 2023 7,252 10 View
Hello, I am doing research on solar panel cooling using phase change material numerically using ANSYS Fluent. How can I convert daily recorded heat flux data into Ansys Fluent UDF format?...
14 March 2023 871 6 View
I want to estimate the annual average total suspended sediment and turbidity of the lake using MODIS image for the period 2000 to 2015 (for both dry and wet season).
25 October 2022 9,640 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
23 July 2024 1,329 5 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
